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Proteintech foxa2
Foxa2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+foxa2/FOXA2+Antibody/pm41917962-50-4-5
Average 94 stars, based on 58 article reviews
foxa2 - by Bioz Stars, 2026-09
94/100 stars

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Related Articles

Incubation:

Article Title: Glycyrrhizic acid combined with human adipose-derived MSCs synergistically alleviates the MPP+/MPTP-induced parkinson's disease by inducing autophagy through PI3K/AKT/HIF-1α pathway.
Article Snippet: .. The sliced sections or treated SH-SY5Y cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton-X-100 in PBS and blocked with 1% bovine serum albumin, 10% normal goat serum, and 0.3% glycine in PBST for 2 h. Subsequently, the sliced sections or cells were incubated with anti-TH (Cell Signaling Technology, USA), anti-Nurr1 (Proteintech, China), anti-MAP2 (Proteintech, China), anti-FOXA2 (Proteintech, China) or anti-LC3B primary antibody (Cell Signaling Technology, USA) at 1:200 in a dark humidity box at 4 °C overnight, then incubated with a secondary antibody (Invitrogen, Alexa Fluor 488/594) goat anti-rabbit IgG (H + L) at 1:500 dilution for 2 h. Finally, after washing, the nuclei were stained with DAPI, and the cells were mounted for imaging. .. Fluorescence was observed using a fluorescence microscope with a 20× objective (final magnification 200×) to analyze TH, Nurr1, MAP2, FOXA2 or LC3B expression and localization in a double-blind manner by two operators (LEICA DMI3000B, Germany).

Article Title: Glycyrrhizic acid combined with human adipose-derived MSCs synergistically alleviates the MPP+/MPTP-induced parkinson’s disease by inducing autophagy through PI3K/AKT/HIF-1α pathway
Article Snippet: .. The sliced sections or treated SH-SY5Y cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton-X-100 in PBS and blocked with 1% bovine serum albumin, 10% normal goat serum, and 0.3% glycine in PBST for 2 h. Subsequently, the sliced sections or cells were incubated with anti-TH (Cell Signaling Technology, USA), anti-Nurr1 (Proteintech, China), anti-MAP2 (Proteintech, China), anti-FOXA2 (Proteintech, China) or anti-LC3B primary antibody (Cell Signaling Technology, USA) at 1:200 in a dark humidity box at 4 °C overnight, then incubated with a secondary antibody (Invitrogen, Alexa Fluor 488/594) goat anti-rabbit IgG (H + L) at 1:500 dilution for 2 h. Finally, after washing, the nuclei were stained with DAPI, and the cells were mounted for imaging. .. Fluorescence was observed using a fluorescence microscope with a 20× objective (final magnification 200×) to analyze TH, Nurr1, MAP2, FOXA2 or LC3B expression and localization in a double-blind manner by two operators (LEICA DMI3000B, Germany).

Staining:

Article Title: Glycyrrhizic acid combined with human adipose-derived MSCs synergistically alleviates the MPP+/MPTP-induced parkinson's disease by inducing autophagy through PI3K/AKT/HIF-1α pathway.
Article Snippet: .. The sliced sections or treated SH-SY5Y cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton-X-100 in PBS and blocked with 1% bovine serum albumin, 10% normal goat serum, and 0.3% glycine in PBST for 2 h. Subsequently, the sliced sections or cells were incubated with anti-TH (Cell Signaling Technology, USA), anti-Nurr1 (Proteintech, China), anti-MAP2 (Proteintech, China), anti-FOXA2 (Proteintech, China) or anti-LC3B primary antibody (Cell Signaling Technology, USA) at 1:200 in a dark humidity box at 4 °C overnight, then incubated with a secondary antibody (Invitrogen, Alexa Fluor 488/594) goat anti-rabbit IgG (H + L) at 1:500 dilution for 2 h. Finally, after washing, the nuclei were stained with DAPI, and the cells were mounted for imaging. .. Fluorescence was observed using a fluorescence microscope with a 20× objective (final magnification 200×) to analyze TH, Nurr1, MAP2, FOXA2 or LC3B expression and localization in a double-blind manner by two operators (LEICA DMI3000B, Germany).

Article Title: Glycyrrhizic acid combined with human adipose-derived MSCs synergistically alleviates the MPP+/MPTP-induced parkinson’s disease by inducing autophagy through PI3K/AKT/HIF-1α pathway
Article Snippet: .. The sliced sections or treated SH-SY5Y cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton-X-100 in PBS and blocked with 1% bovine serum albumin, 10% normal goat serum, and 0.3% glycine in PBST for 2 h. Subsequently, the sliced sections or cells were incubated with anti-TH (Cell Signaling Technology, USA), anti-Nurr1 (Proteintech, China), anti-MAP2 (Proteintech, China), anti-FOXA2 (Proteintech, China) or anti-LC3B primary antibody (Cell Signaling Technology, USA) at 1:200 in a dark humidity box at 4 °C overnight, then incubated with a secondary antibody (Invitrogen, Alexa Fluor 488/594) goat anti-rabbit IgG (H + L) at 1:500 dilution for 2 h. Finally, after washing, the nuclei were stained with DAPI, and the cells were mounted for imaging. .. Fluorescence was observed using a fluorescence microscope with a 20× objective (final magnification 200×) to analyze TH, Nurr1, MAP2, FOXA2 or LC3B expression and localization in a double-blind manner by two operators (LEICA DMI3000B, Germany).

Imaging:

Article Title: Glycyrrhizic acid combined with human adipose-derived MSCs synergistically alleviates the MPP+/MPTP-induced parkinson's disease by inducing autophagy through PI3K/AKT/HIF-1α pathway.
Article Snippet: .. The sliced sections or treated SH-SY5Y cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton-X-100 in PBS and blocked with 1% bovine serum albumin, 10% normal goat serum, and 0.3% glycine in PBST for 2 h. Subsequently, the sliced sections or cells were incubated with anti-TH (Cell Signaling Technology, USA), anti-Nurr1 (Proteintech, China), anti-MAP2 (Proteintech, China), anti-FOXA2 (Proteintech, China) or anti-LC3B primary antibody (Cell Signaling Technology, USA) at 1:200 in a dark humidity box at 4 °C overnight, then incubated with a secondary antibody (Invitrogen, Alexa Fluor 488/594) goat anti-rabbit IgG (H + L) at 1:500 dilution for 2 h. Finally, after washing, the nuclei were stained with DAPI, and the cells were mounted for imaging. .. Fluorescence was observed using a fluorescence microscope with a 20× objective (final magnification 200×) to analyze TH, Nurr1, MAP2, FOXA2 or LC3B expression and localization in a double-blind manner by two operators (LEICA DMI3000B, Germany).

Article Title: Glycyrrhizic acid combined with human adipose-derived MSCs synergistically alleviates the MPP+/MPTP-induced parkinson’s disease by inducing autophagy through PI3K/AKT/HIF-1α pathway
Article Snippet: .. The sliced sections or treated SH-SY5Y cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton-X-100 in PBS and blocked with 1% bovine serum albumin, 10% normal goat serum, and 0.3% glycine in PBST for 2 h. Subsequently, the sliced sections or cells were incubated with anti-TH (Cell Signaling Technology, USA), anti-Nurr1 (Proteintech, China), anti-MAP2 (Proteintech, China), anti-FOXA2 (Proteintech, China) or anti-LC3B primary antibody (Cell Signaling Technology, USA) at 1:200 in a dark humidity box at 4 °C overnight, then incubated with a secondary antibody (Invitrogen, Alexa Fluor 488/594) goat anti-rabbit IgG (H + L) at 1:500 dilution for 2 h. Finally, after washing, the nuclei were stained with DAPI, and the cells were mounted for imaging. .. Fluorescence was observed using a fluorescence microscope with a 20× objective (final magnification 200×) to analyze TH, Nurr1, MAP2, FOXA2 or LC3B expression and localization in a double-blind manner by two operators (LEICA DMI3000B, Germany).

Chromatin Immunoprecipitation:

Article Title: The Histone Methyltransferase KMT2D is a Critical Mediator of Lineage Plasticity and Therapeutic Response in Castration Resistant Prostate Cancer
Article Snippet: .. The membranes were probed with the following primary antibodies: anti-KMT2D (Fortis LifeSciences Cat# A300-BL1185, RB X-ALR, 1:500), phospho-KMT2D (S1331) (Eurogentec; Custom, 1:250), anti-AR [D6F11] XP (Cell Signaling Technology Cat# 5153, RRID:AB_10691711, 1:2000), anti-FOXA1 [E7E8W] (Cell Signaling Technology Cat# 53528, RRID:AB_2799438, 1:1000), anti-FOXA2 (Proteintech Cat# 22474–1-AP, RRID:AB_2879110, 1:1000), anti-V5 [D3H8Q] (Cell Signaling Technology Cat# 13202, RRID: AB_2687461, 1:1000), anti-synaptophysin [YE269] (Abcam Cat# ab32127, RRID: AB_2286949 1:1000), anti-beta actin (Cell Signaling Technology Cat# 4967, RRID: AB_330288, 1:2000), anti-vinculin (Cell Signaling Technology Cat# 4650, RRID: AB_10559207, 1:1000), Histone H3 (mono methyl K4) antibody- ChIP Grade (Abcam Cat# ab8895, RRID: AB_306847, 1:500), Histone H3 [D2B12] (Cell Signaling Technology Cat# 4620, RRID: AB_1904005, 1:1000), anti-keratin 5 (BioLegend Cat# 905504, RRID:AB_2616956, 1:10000), purified anti-keratin 8 (BioLegend Cat# 904801, RRID: AB_2565043, 1:5000), Vimentin [D21H3] XP (Cell Signaling Technology Cat# 5741, RRID: AB_10695459, 1:1000), ECL TM Anti-Rabbit IgG HRP- linked antibody (from donkey) (Cat# NA934V; Lot 17010251, RRID: AB_772206, 1:4000), Anti-mouse IgG HRP- linked antibody (Cell Signaling Technology Cat# 7076, RRID: AB_330924, 1:2000). .. Immunohistochemistry and multiplex immunofluorescence of the organoids and cell lines were performed using Ventana Discovery Ultra Auto Stainer, using published Ventana standard protocols.

Purification:

Article Title: The Histone Methyltransferase KMT2D is a Critical Mediator of Lineage Plasticity and Therapeutic Response in Castration Resistant Prostate Cancer
Article Snippet: .. The membranes were probed with the following primary antibodies: anti-KMT2D (Fortis LifeSciences Cat# A300-BL1185, RB X-ALR, 1:500), phospho-KMT2D (S1331) (Eurogentec; Custom, 1:250), anti-AR [D6F11] XP (Cell Signaling Technology Cat# 5153, RRID:AB_10691711, 1:2000), anti-FOXA1 [E7E8W] (Cell Signaling Technology Cat# 53528, RRID:AB_2799438, 1:1000), anti-FOXA2 (Proteintech Cat# 22474–1-AP, RRID:AB_2879110, 1:1000), anti-V5 [D3H8Q] (Cell Signaling Technology Cat# 13202, RRID: AB_2687461, 1:1000), anti-synaptophysin [YE269] (Abcam Cat# ab32127, RRID: AB_2286949 1:1000), anti-beta actin (Cell Signaling Technology Cat# 4967, RRID: AB_330288, 1:2000), anti-vinculin (Cell Signaling Technology Cat# 4650, RRID: AB_10559207, 1:1000), Histone H3 (mono methyl K4) antibody- ChIP Grade (Abcam Cat# ab8895, RRID: AB_306847, 1:500), Histone H3 [D2B12] (Cell Signaling Technology Cat# 4620, RRID: AB_1904005, 1:1000), anti-keratin 5 (BioLegend Cat# 905504, RRID:AB_2616956, 1:10000), purified anti-keratin 8 (BioLegend Cat# 904801, RRID: AB_2565043, 1:5000), Vimentin [D21H3] XP (Cell Signaling Technology Cat# 5741, RRID: AB_10695459, 1:1000), ECL TM Anti-Rabbit IgG HRP- linked antibody (from donkey) (Cat# NA934V; Lot 17010251, RRID: AB_772206, 1:4000), Anti-mouse IgG HRP- linked antibody (Cell Signaling Technology Cat# 7076, RRID: AB_330924, 1:2000). .. Immunohistochemistry and multiplex immunofluorescence of the organoids and cell lines were performed using Ventana Discovery Ultra Auto Stainer, using published Ventana standard protocols.

Western Blot:

Article Title: FOXA2 regulated by HDAC3-mediated deacetylation attenuates neuropathic pain by modulating microglial lipid metabolism and synaptic pruning dysregulation
Article Snippet: Subsequently, Protein A/G Magnetic Beads (Beyotime, China) were added to the mixture and incubated at room temperature for 1 h. The beads were washed thoroughly with lysis buffer, and the bound proteins were eluted by resuspension in SDS sample buffer and boiling for 10 min. .. Finally, the eluted proteins were analyzed by western blotting using the following primary antibodies: anti-acetylated-lysine (1:1000, Cell Signaling Technology, USA), anti-FOXA2 (1:1000, Proteintech, China), and anti-HDAC3 (1:1000, Proteintech, China). .. GraphPad Prism 9.0 was used to perform statistical tests and all data are shown as the mean ± S.E.M. from at least three independent experiments.

Article Title: FOXA2 regulated by HDAC3-mediated deacetylation attenuates neuropathic pain by modulating microglial lipid metabolism and synaptic pruning dysregulation.
Article Snippet: Subsequently, Protein A/G Magnetic Beads (Beyotime, China) were added to the mixture and incubated at room temperature for 1 h. The beads were washed thoroughly with lysis buffer, and the bound proteins were eluted by resuspension in SDS sample buffer and boiling for 10 min. .. Finally, the eluted proteins were analyzed by western blotting using the following primary antibodies: anti-acetylated-lysine (1:1000, Cell Signaling Technology, USA), anti-FOXA2 (1:1000, Proteintech, China), and anti-HDAC3 (1:1000, Proteintech, China). .. GraphPad Prism 9.0 was used to perform statistical tests and all data are shown as the mean ± S.E.M. from at least (See figure on previous page.)



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